An-Najah National University

Suleiman Ibrahim Al-Khalil

Biomedical Scienctist,FIBMS,CCHEM MRSC,

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  • Wednesday, August 10, 2005
  • Antibacterial Activity of {Khellin} in Different Crude Extracts From
  • Published at:The First Palestinian Medical and Health Conference, August 2005
  • Suleiman Al Khalil
    Lübna Al- Sharif
    An-Najah National University

    The objectives of this study on Ammi visnaga fruits were to extract the most active ingredient” Khellin” in different methods, conditions & forms to obtain different crude extracts with different properties that contain Khellin in various proportions.
    We extracted khellin in impure form by n-hexane: MeOH procedure; in which we changed n-Hexane proportion & time of mixing with plant powder, sterilized in 10% DMSO & gave good results at Broth dilution method of fresh preparations.
    Also, we aimed from this project: authentication these results by detecting presence of khellin on TLC, that showed the presence of Khellin in high proportions at different crude preparations, but in impure form. The most advanced eluient was
    EtOASc: CHCL3 60:40 v/v in comparison to EtOAc-eluent alone (although it appeared in conjunction to visnagin).
    These results should encourage continuous researches & gather the efforts to be directed towards the importance of herbal medicine in our area due to: high number of herbal plants & great diversity of active ingredients (Like Khellin in A.visnaga), which it contains to help in many ailments and diseases treatment at this century.

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Suleiman Ibrahim Al-Khalil
Biomedical Scientist,
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