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  • Monday, February 9, 2009
  • Identification of lactic acid bacteria in Moroccan raw milk and traditionally fermented skimmed milk 'lben'.
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  • Ouadghiri, M., Vancanneyt, M., Vandamme, P., Naser M. S., Gevers, D., Lefebvre, K., Swings, J. and Amar, M. 

     

     

    Thirty-seven samples were collected from different farms.

    A total of 146 LAB were isolated and subjected to (GTG)

     

    5-PCR analysis. Comparison

    of the profiles with data available at the Moroccan Coordinated Collections

    of Micro-organisms allowed identification of 85 isolates. The remaining

    61 were subjected to SDS-PAGE analysis of whole cell proteins. Comparison of

    the profiles with data available at the Belgian Coordinated Collections of

    Micro-organisms allowed identification of 43 isolates. Several of the remaining

    18 isolates exhibited identical protein electrophoretic fingerprints. Therefore,

    eight representatives of them were subjected to partial

     

    pheS gene sequencing

    which allowed identification of all remaining isolates. In raw milk, six genera

    were found while in ‘lben’, three were found. This is the first report of

     

    Leuconostoc

    kimchii

     

    in dairy products.

     LAB diversity was established using a stepwise polyphasic identifi-

    cation approach. It used the expertise of both research bodies involved in this

    study and proved to be cost-effective for the identification of all isolates.

    Significance and Impact of the Study:

     

    To establish LAB diversity in Moroccan

    dairy products which could be a source of strains with specific properties.

     
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Sabri Mahmoud Sabri Naser
Biotechnology (Molecular Biology and Bioinformatics)
 
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