- Tuesday, March 23, 2010
- Published at:Not Found
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Molecular identification of lactic acid bacteria Enterococcus, Lactobacillus and Streptococcus based on pheS, rpoA and atpA multilocus sequence analysis (MLSA)
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- Monday, February 9, 2009
- Published at:Not Found
- Ouadghiri, M., Vancanneyt, M., Vandamme, P., Naser M. S., Gevers, D., Lefebvre, K., Swings, J. and Amar, M.
Thirty-seven samples were collected from different farms.
A total of 146 LAB were isolated and subjected to (GTG)
5-PCR analysis. Comparison
of the profiles with data available at the Moroccan Coordinated Collections
of Micro-organisms allowed identification of 85 isolates. The remaining
61 were subjected to SDS-PAGE analysis of whole cell proteins. Comparison of
the profiles with data available at the Belgian Coordinated Collections of
Micro-organisms allowed identification of 43 isolates. Several of the remaining
18 isolates exhibited identical protein electrophoretic fingerprints. Therefore,
eight representatives of them were subjected to partial
pheS gene sequencing
which allowed identification of all remaining isolates. In raw milk, six
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- Friday, January 30, 2009
- Published at:Not Found
- Sabri M. Naser, Peter Dawyndt, Bart Hoste, Dirk Gevers, Katrien Vandemeulebroecke, Ilse Cleenwerck, Marc Vancanneyt& Jean Swings
The aim of our study was to evaluate the use of the phenylalanyl-tRNA synthase alpha subunit (pheS) and the RNA polymerase alpha subunit (rpoA) partial gene sequences for species identification of Lactobacillus. Two hundred and one strains representing the validly named species (98) and their subspecies (17) were examined. The pheS gene sequence analysis provides an interspecies gap, which normally exceeds 10% divergence and an intraspecies variation up to 3%. The rpoA gene sequences revealed a somewhat lower resolution with an interspecies gap normally exceeding 5% and an intraspecies variation up to 2%. The combined use of pheS and rpoA gene sequences offers a reliable identification system for nearly all Lactobacillus species. The pheS and rpoA gene sequences provide a powerful tool for detection of the potential novel Lactobacillus species and synonymous tax
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- Friday, January 30, 2009
- Published at:Not Found
- Rademaker, J. L.W., Herbet, H., Starrenburg, M. J.C., Naser, S. M., Gevers, D., Kelly, W. J., Hugenholtz, J., Swings, J., and van Hylckama Vlieg, J. E.T
The diversity of a collection of 102 lactococcus isolates including 91 Lactococcus lactis isolates of dairy and nondairy origin was explored using partial small subunit rRNA gene sequence analysis and limited phenotypic analyses. A subset of 89 strains of L. lactis subsp. cremoris and L. lactis subsp. lactis isolates was further analyzed by (GTG)5-PCR fingerprinting and a novel multilocus sequence analysis (MLSA) scheme. Two major genomic lineages within L. lactis were found. The L. lactis subsp. cremoris type-strain-like genotype lineage included both L. lactis subsp. cremoris and L. lactis subsp. lactis isolates. The other major lineage, with a L. lactis subsp. lactis type-strain-like genotype, comprised L. lactis subsp. lactis isolates only. A novel third genomic lineage represented two L. lactis subsp. lactis isolates of nondairy origin. Th
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- Friday, January 30, 2009
- Published at:Not Found
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M. Kawalec
, J. Ke¸dzierska, A. Gajda, E. Sadowy, J. We¸grzyn, S. M. Naser, A. B. Skotnicki ,M. Gniadkowski and W. Hryniewicz
A mixed outbreak caused by vancomycin-resistant
Enterococcus raffinosus and Enterococcus faecium
carrying the
vanA gene was analysed. The outbreak occurred in a large hospital in Poland and affected
27 patients, most of whom were colonised, in three wards, including the haematology unit. The
E. raffinosus
isolates had a high-level multiresistant phenotype and were initially misidentified as
Enterococcus avium
; their unambiguous identification was provided by multilocus sequence analysis. The
molecular investigation demonstrated the clonal character of the
E. raffinosus outbreak and the
polyclonal structure of the
E. faecium isolates. All of the isolates carried the same Tn1546-like element
containing an IS
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RSS
Sabri Mahmoud Sabri Naser