- Thursday, February 19, 2009
- Birler, S., Pabuccuoglu, S., Alkan, S., Atalla, H., Ileri, I.K.: Pronuclear Development of in vitro Fertilized Sheep Oocytes. Reproduction, Abstract Series, 27, 8. Society for the Study of Fertility, Annual Conference, 8-11 July, Cambridge-England, 2001
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- Sunday, February 15, 2009
- Published at:Not Found
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The cryopreservation of mammalian embryos has become a well-established procedure. However, unfertilized oocytes are only rarely cryopreserved due to their sensitivity to environmental factors. In this study, CB6, F1 mouse oocytes (n=1321 unfertilized) were vitrified after exposure to VS1 medium. After thawing at 37°C for 20 second, oocytes were fertilized in vitro. The proportion of blastocyst rates at 96 h post insemination were untreated (group A), VS1 exposed (group B) and vitrification (group C) groups were ; 77.08%, 62.74% and 31.42% respectively. These results show that mouse oocytes can be frozen successfully in VS1 medium by vitrification technique.
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- Thursday, January 1, 2004
- Published at:Turk J Vet Anim Sci, 28 (2004) 831-835
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Abstract:
This study was carried out to determine the growth, survival rate and some body measurements until weaning of lamb sproduced by the in vitro production (IVP) method in comparison with those of lambs produced by artificial insemination. The lambs in the experimental group were produced by the transfer of IVP embryos developed to the blastocyst stage after the in vitro maturation and fertilisation of oocytes obtained from the ovaries of slaughtered ewes.
The results of this study showed that the lambs produced by the IVP technique had a higher growth rate until weaning than did the lambs in the control group (P < 0.05). There were no significant differences between the experimental and control groups in terms of survival rate and body measurements.
It was concluded that the IVP technique, which provides significant improvements in population genetics, could be successfully applie
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- Wednesday, January 1, 2003
- Published at:Turk J Vet Anim Sci, 27 (2003) 1409-1412
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Abstract:
The aim of the present study was to investigate the effects of gonadotrophin and Bovine Serum Albumin (BSA)s upplemented Synthetic Oviduct Fluid (SOF) and HamÕs F-10 media on the in vitro maturation of cat oocytes. Oocytes collected from spayed stray queens served as the material of the study. The ovaries were brought to the laboratory within 2 h in PBS solution at 38 C. Recovered oocytes were divided into two groups (Group 1: SOF + 0,4% BSA + 10 µg/ml FSH + 10 µg/ml LH, group 2: Ham's F-10 + 0,4% BSA + 10 µg/ml FSH + 10 µg/ml LH) and left for maturation in an incubator at 38.5 ¡C for 48 h under atmosphere containing 5% O2, 5% CO2 and 90 % N2 and almost 100% humidity. Oocytes were then fixed and stained. The maturation status of the oocytes was evaluated under a phase-contrast microscope at x400 magnification. Data were compared by
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- Tuesday, January 1, 2002
- Published at:Turk J Vet Anim Sci, 26 (2002) 1421-1426
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Abstract:
The objective of the present study was to transfer sheep embryos produced in vitro to recipient ewes. Ovaries were taken
from slaughtered ilvircik ewes and transferred to the laboratory in phosphate buffered saline (PBS) at 30-35 ¼C. The cumulusoocyte complexes were obtained by slicing and washing 1-6 mm diameter follicles and matured for 24 h in medium 199 supplemented with sodium pyruvate, follicle stimulating hormone (FSH), luteinizing hormone (LH) and 10% fetal calf serum (FCS) at 38.5 C under 5% CO
2
in humidified atmosphere. Fresh semen was collected from three kivircik rams, pooled and prepared for
in vitro fertilization by the percoll-gradient method. Matured oocytes were transferred into synthetic oviduct fluid (SOF) based fertilization medium supplemented with 2% sheep oestrous serum (SES)
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Hatem Abdelateef Atalla