http://blogs.najah.edu/author/hatem-2381 An-Najah Blogs :: Dr. Hatem Atalla en-us Thu, 18 Apr 2024 10:20:47 IDT Thu, 18 Apr 2024 10:20:47 IDT [email protected] [email protected] http://blogs.najah.edu/staff/hatem-2381/article/IN-VITRO-FERTILIZATION-OF-VITRIFIED-MOUSE-OOCYTESPublished Articles The cryopreservation of mammalian embryos has become a well-established procedure However unfertilized oocytes are only rarely cryopreserved due to their sensitivity to environmental factors In this study CB6 F1 mouse oocytes n=1321 unfertilized were vitrified after exposure to VS1 medium After thawing at 37C for 20 second oocytes were fertilized in vitro The proportion of blastocyst rates at 96 h post insemination were untreated group A VS1 exposed group B and vitrification group C groups were ; 7708 6274 and 3142 respectively These results show that mouse oocytes can be frozen successfully in VS1 medium by vitrification technique http://blogs.najah.edu/staff/hatem-2381/article/A-Comparative-Study-on-the-Growth-and-Survival-Characteristics-ofPublished Articles Abstract: This study was carried out to determine the growth survival rate and some body measurements until weaning of lamb sproduced by the in vitro production IVP method in comparison with those of lambs produced by artificial insemination The lambs in the experimental group were produced by the transfer of IVP embryos developed to the blastocyst stage after the in vitro maturation and fertilisation of oocytes obtained from the ovaries of slaughtered ewes The results of this study showed that the lambs produced by the IVP technique had a higher growth rate until weaning than did the lambs in the control group P 005 There were no significant differences between the experimental and control groups in terms of survival rate and body measurements It was concluded that the IVP technique which provides significant improvements in population genetics could be successfully applied in sheep breeding and could be of help in the improvement of the meat produced from lambs Key Words: Growth in vitro production kivircik sheep breed survival rate http://blogs.najah.edu/staff/hatem-2381/article/Studies-on-the-in-vitro-Maturation-of-Cat-OocytesPublished Articles Abstract: The aim of the present study was to investigate the effects of gonadotrophin and Bovine Serum Albumin BSAs upplemented Synthetic Oviduct Fluid SOF and Hams F-10 media on the in vitro maturation of cat oocytes Oocytes collected from spayed stray queens served as the material of the study The ovaries were brought to the laboratory within 2 h in PBS solution at 38 C Recovered oocytes were divided into two groups Group 1: SOF 04 BSA 10 gml FSH 10 gml LH group 2: Hams F-10 04 BSA 10 gml FSH 10 gml LH and left for maturation in an incubator at 385 C for 48 h under atmosphere containing 5 O2 5 CO2 and 90 N2 and almost 100 humidity Oocytes were then fixed and stained The maturation status of the oocytes was evaluated under a phase-contrast microscope at x400 magnification Data were compared by using Students t-test Group 1 had 209 oocytes and group 2 had 207: a total of 416 In group 1 5406 113209 and in group 2 2900 60207 of the oocytes reached the M II stage The difference between these values was statistically significant At the end of the study it was concluded that SOF medium was superior to Hams F-10 medium for the in vitro maturation of cat oocytes These results also showed that a satisfactory background for in vitro fertilization studies of cat oocytes has been established Key Words: Cat oocyte in vitro maturation medium http://blogs.najah.edu/staff/hatem-2381/article/Transfer-of-In-Vitro-Produced-Sheep-EmbryosPublished Articles Abstract: The objective of the present study was to transfer sheep embryos produced in vitro to recipient ewes Ovaries were taken from slaughtered ilvircik ewes and transferred to the laboratory in phosphate buffered saline PBS at 30-35 frac14;C The cumulusoocyte complexes were obtained by slicing and washing 1-6 mm diameter follicles and matured for 24 h in medium 199 supplemented with sodium pyruvate follicle stimulating hormone FSH luteinizing hormone LH and 10 fetal calf serum FCS at 385 C under 5 CO 2 in humidified atmosphere Fresh semen was collected from three kivircik rams pooled and prepared for in vitro fertilization by the percoll-gradient method Matured oocytes were transferred into synthetic oviduct fluid SOF based fertilization medium supplemented with 2 sheep oestrous serum SES and co-incubated with semen 08 x 10 6 spermatozoonml for 20-21 h After fertilization presumptive zygotes were transferred into SOF medium and incubated for 8 days under an atmosphere of 5 CO 2 5 O2 and 90 N2 at 385 frac14;C Glucose 15 mM was added to the culture medium on day 4 In culture embryos were checked for cleavage and embryo development on days 4 and 8 respectively A total of 534 oocytes were inseminated in vitro 407 762 cleaved and 119 292 and 69 169 reached the morula and blastocyst stages respectively Forty days after the transfer of 40 blastocysts into the uteri of 17 recipient ewes pregnancy was detected in 10 ewes 588 by ultrasonography and 7 ewes gave birth to 8 live lambs The other 3 ewes which had 7 fetuses could not have live offspring According to the transferred embryos the implantation rate was 375 and the live lamb rate was 200 Key Words: Sheep in vitro production embryo transfer http://blogs.najah.edu/staff/hatem-2381/article/Development-of-In-Vitro-Derived-Sheep-Embryos-to-the-Blastocyst-StagePublished ArticlesThe objective of the present study was to mature and fertilize primary sheep oocytes in vitro and to develop them to the blastocyst stage Ovaries from slaughteredkivircik ewes were used The ovaries were transported to the laboratory in a vacuum flask in which 30-35 C PBS phosphate buffered saline was included The cumulus-oocyte complexes were collected by rupturing and washing the follicle wall with oocyte washing medium They were then selected under a stereo microscope and maturated for 23-24 hours in medium 199 supplemented with sodium pyruvate follicle stimulating hormone FSH luteinizing hormone LH and 10 fetal calf serum FCS under 5 CO 2 atmosphere and at 385 C temperature Semen collected and pooled from three Kivircik rams by electro-ejaculation and prepared for in vitro fertilization by Percoll-gradient method was incubated for 20-21 hours with oocytes in BSOF medium supplemented with 2 sheep estrous serum SES 08x106spermatozoonml Presumptive zygotes were cultured in SOF medium for 8 days in synthetic oviduct medium SOF under an atmosphere of 5 CO 25 O2 and 90 N2 Glucose 15mM was added to the culture medium on Day 4 Out of 171 oocytes 151 cleaved 883 Fifty-six morulae 371 and 14 blastocysts 93 were obtained from these in vitro derived embryos Key Words: Sheep in vitro fertilization in vitro culture development to blastocyst stage http://blogs.najah.edu/staff/hatem-2381/article/Pronuclear-Development-of-in-vitro-Fertilized-Sheep-OocytesGeneral PostsBirler S Pabuccuoglu S Alkan S Atalla H Ileri IK: Pronuclear Development of in vitro Fertilized Sheep Oocytes Reproduction Abstract Series 27 8 Society for the Study of Fertility Annual Conference 8-11 July Cambridge-England 2001