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  • Saturday, January 1, 1994
  • Determination of nanomolar levels of guanine by differential-pulse adsorptive cathodic stripping voltammetry of its copper(II) complex
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  • Guanine is determined at the 5.0×10–10 –2.0×10–7 mol/l level by differential-pulse adsorptive stripping voltammetry at a hanging mercury drop electrode using the reduction peak of its copper (II) complex at –0.21 V vs. Ag-AgCl electrode. The optimum analytical conditions were found to be Britton-Robinson buffer solution (pH 4.8), an accumulation potential of 0.0 V and an accumulation time of 3 min. Under these conditions, the detection limit is 5.0×10–10 mol/l and the relative standard deviation 2.6% for 1.0×10–7 mol/l guanine. The method is compared with the previous voltammetric methods. The presence of some purine derivatives does not interfere.


    Fresenius' Journal of Analytical Chemistry, Volume 348, Number 11 / November, 1994


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Raqi Moh'd Shubitah
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