Eleven winter barley cultivars from Palestine screened for their resistance to powdery mildew in the field and under controlled conditions while, two types of resistance were identified. Three accessions showed low infection type (IT) at seedling stage with low disease severity (DS) in the mature plant. Six other lines showed low DS and low infection frequency (IF) with no macroscopically visible necrosis. These resistant accessions were selected and selfed for future studies.