- Saturday, March 2, 2019
- Published at:Revue Méd. Vét.,
- This study was conducted to evaluate the protective effects of cholesterol pre-incubation on ovine oocytes exposed to cold stress and vitrification. In the first experiment, cumulus oocyte complexes harvested from ovaries of slaughtered ewes were exposed to 2 mg/ml cholesterol loaded methyl-ßcyclodextrin (CLC) in maturation medium for 2, 4 or 24 h. Oocytes were then exposed to cold stress at 4°C for 30 min, and the viability and chromosome abnormality of oocytes were evaluated. Our results showed no significant differences between treatment groups in regarding to oocyte viability or ooplasm integrity. However, oocytes exposed to cold stress without preincubation with CLC had significantly higher chromatin dispersion (P<0.05) than pre-incubated oocytes with CLC. In second experiment, oocytes were exposed to 2 mg/ml CLC in maturation medium for 2 h and vitrified after maturation in conventional straws (0.25 ml; CS) or in open pulled straws (OPS). Osmotic stress of vitrification media to oocytes was
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- Friday, January 5, 2018
- Published at: Iraqi Journal of Veterinary Sciences, Vol. 31, No. 2, 2017
- This investigation of Q-fever or Coxiellosis was undertaken to study the presence and the infection rate of C.burnetii infection in reproductively active rams in the Northern Palestine, where most of the sheep herds are located. In all, 2806 samples which collected from active rams during the reproductive season from herds in five cities (Jenin, Jericho, Nablus, Tulkarm, and Tubas). Sera were tested by ID Screen® Q Fever Indirect Multi-species enzyme-linked immunosorbent assay (ELISA) for detection of C. burnetii immunoglobulin G (IgG). A total of 28.1 % of ram sera were positive for C. burnetii IgG. There was no significant difference between the four location cities studied, However, a significantly low difference was observed in the Tubas city (P<0.05). The highest infection rate was detected in rams of Tulkarm (29.8%), followed by Nablus (29.5%), Jericho (28.4%), Jenin (28.2%) and Tubas (16.1%). Moreover, at the farm level, 73.3% had at least one seropositive animal. It had been concluded that a hi
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- Tuesday, December 19, 2017
- Published at:Front. Microbiol., 15 December 2017
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- Monday, August 21, 2017
- Published at:Revue de Médecine Vétérinaire
- Chlamydophila abortus is an important pathogen in human and a variety of animals. Ovine chlamydiosis (also known as Enzootic Abortion of Ewes (EAE)) is a venereal transmitted disease associated with late abortion and economic impact in sheep production. There is a lack of information about its status in Palestine, in which sheep are the most abundant farm animal that has a traditional and economic importance. Particularly, the infection rate in male is not well documented. In the present study, 2608 blood samples were collected from active reproductive rams between April and June 2016 in five Palestinian cities (Jenin, Nablus, Jericho, Tulkarm, and Tubas). Sera were tested by ID Screen® Chlamydophila abortus Indirect Multi-species enzyme-linked immunosorbent assay (ELISA) kit for detection of C. abortus immunoglobulin G (IgG) (IDvet, France). At the individual level, among 2608 samples analyzed, 385 (13.7%) were seropositive for C. abortus. The chi-square analysis was used to evaluate the risk factors ass
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- Saturday, May 20, 2017
- Published at:Scientific Journal of Veterinary Advances
- Contagious agalactiae caused by Mycoplasma agalctiae (M. agalctiae) implicated for important losses in small ruminant due to mortality, decreased milk production, and cost of treatment and prevention. There is a lack of information about the disease status in Palestine. In this study, a survey of M. agalctiae antibodies in sheep blood from Jericho city near the Jordan River in the eastern Palestine was conducted. A total of 611 randomly selected sheep of different ages were investigated from different farms in two regions in Jericho city during the period from December 2016 to March 2017 to. Blood samples were collected and serum was subjected to serologic examination for detection of antibodies against M. agalctiae major membrane lipoprotein of (p48) using enzyme-linked immunosorbent assay (ELISA, CHECKIT, IDEXX). The overall M. agalctiae seroprevalence rate was 11.5%. Regarding the distribution of the disease in the investigated regions in Jericho city; the seroprevalence rate of M. agalctiae in sheep was 1
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Ibrahim M. Alzuheir