An-Najah National University

Maher Abdel Razzak Abu-Eid Natsheh


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  • Tuesday, February 1, 1994
  • Plumbagin, a Naturally Occurring Naphthoquinone: Its Isolation, Spectrophotometric Determination in Roots, Stems, and Leaves in Plumbago Europaea L.
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  • DOI: 10.1080/00387019408007247

    Published in:  Spectroscopy Letters, Volume 27, Issue 4 May 1994 , pages 409 - 416


    Authors: Mohammed A. Al-Nuri a;  Mohammed A. Hannoun a;  Nidal A. Zatar a;  Maher A. Abu-Eid a;  Waheed J. Al-Jondi a;  Ahmad I. Hussein a; Mohammed S. Ali-Shtayeh b


    A new method for isolation and spectrophotometric determination of plumbagin is presented. Plumbagin was isolated by thin layer chromatography (TLC) and column chromatography (CC) techniques, as an orange tinged yellow long crystalline substances. Plumbagin exhibits two absrop-tion maxima at 410 and 510 nm. Stability of the color, pKa value, and the effect of pH were studied. Beer's law is obeyed over the range 0.9-45 ppm. The method is applied to the determination of plumbagin in roots, stems, and leaves of Plumbago europaea L. plant. 
    Keywords: Plumbagin; Isolation; Spectrophotometric Determination; Roots; Stems; Leaves; Plumbago Europaea L; pKa; Acid-Base Indicator.



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Maher Abdel Razzak Abu-Eid Natsheh
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