An-Najah Blogs :: Adnan Fayyad http://blogs.najah.edu/author/ss226228 An-Najah Blogs :: Adnan Fayyad en-us Sun, 17 Nov 2019 11:37:39 IST Sun, 17 Nov 2019 11:37:39 IST webmaster@najah.edu webmaster@najah.edu PCR-restriction endonuclease analysis of Mycobacterium avium subsp. paratuberculosis isolates from goats, sheep, and cattle in Jordanhttp://blogs.najah.edu/staff/ss226228/article/PCR-restriction-endonuclease-analysis-of-Mycobacterium-avium-subsp-paratuberculosis-isolates-from-goats-sheep-and-cattle-in-JordanPublished Articles Mycobacterium avium subsp paratuberculosis MAP isolated from animals in Jordan In this study we investigated 150 fecal samples from sheep goats and cattle for the presence of paratuberculosis using bacterial culture and polymerase chain reaction PCR-RFLP analysis of insertion sequence IS 1311 Analysis of the results revealed that genotypic information from sheep goat and cattle could classify them into cattle or sheep strains All culture isolates from cattle 125 of the isolates from sheep and 50 of the isolates from goats were cattle strain while 875 of the isolates from sheep and 50 of the isolates from goats were sheep strain Sequencing of the IS 1311 268 bp PCR product from the three animal species confirmed the different MAP patterns Paratuberculosis is an endemic disease and induces high economical losses in Jordan There is no information available on genotypic variation of