An-Najah Blogs :: Ibrahim Alzuheir An-Najah Blogs :: Ibrahim Alzuheir en-us Wed, 26 Feb 2020 22:46:21 IST Wed, 26 Feb 2020 22:46:21 IST Effect of cholesterol loaded methyl-β-cyclodextrin on ovine oocytes during chilling and vitrification ArticlesThis study was conducted to evaluate the protective effects of cholesterol pre-incubation on ovine oocytes exposed to cold stress and vitrification In the first experiment cumulus oocyte complexes harvested from ovaries of slaughtered ewes were exposed to 2 mgml cholesterol loaded methyl-cyclodextrin CLC in maturation medium for 2 4 or 24 h Oocytes were then exposed to cold stress at 4C for 30 min and the viability and chromosome abnormality of oocytes were evaluated Our results showed no significant differences between treatment groups in regarding to oocyte viability or ooplasm integrity However oocytes exposed to cold stress without preincubation with CLC had significantly higher chromatin dispersion P005 than pre-incubated oocytes with CLC In second experiment oocytes were exposed to 2 mgml CLC in maturation medium for 2 h and vitrified after maturation in conventional straws 025 ml; CS or in open pulled straws OPS Osmotic stress of vitrification media to oocytes was also evaluated Oocytes pre-treated with CLC had a significantly higher viability rate and ooplasm integrity than non-treated oocytes after vitrification in OPS P 005 The findings of this study also show that CLC pre-incubation of ovine oocytes reduce the chromosome degeneration after vitrification in both OPS and CSSerosurvey of Q fever in active reproductive rams in northern Palestine ArticlesThis investigation of Q-fever or Coxiellosis was undertaken to study the presence and the infection rate of Cburnetii infection in reproductively active rams in the Northern Palestine where most of the sheep herds are located In all 2806 samples which collected from active rams during the reproductive season from herds in five cities Jenin Jericho Nablus Tulkarm and Tubas Sera were tested by ID Screen Q Fever Indirect Multi-species enzyme-linked immunosorbent assay ELISA for detection of C burnetii immunoglobulin G IgG A total of 281 of ram sera were positive for C burnetii IgG There was no significant difference between the four location cities studied However a significantly low difference was observed in the Tubas city P005 The highest infection rate was detected in rams of Tulkarm 298 followed by Nablus 295 Jericho 284 Jenin 282 and Tubas 161 Moreover at the farm level 733 had at least one seropositive animal It had been concluded that a high infection rate was detected in rams of Northern Palestine at both individual and herd level Therefore Q fever could be responsible for considerable numbers of ovine abortions in Palestine as well as of public health significance Hereby control programs should be advisedIdentification of the Receptor and Cellular Ortholog of the Marek`s Disease Virus (MDV) CXC Chemokine Articles Detection of Chlamydophila abortus antibody in active reproductive rams in sheep herds in northern Palestine ArticlesChlamydophila abortus is an important pathogen in human and a variety of animals Ovine chlamydiosis also known as Enzootic Abortion of Ewes EAE is a venereal transmitted disease associated with late abortion and economic impact in sheep production There is a lack of information about its status in Palestine in which sheep are the most abundant farm animal that has a traditional and economic importance Particularly the infection rate in male is not well documented In the present study 2608 blood samples were collected from active reproductive rams between April and June 2016 in five Palestinian cities Jenin Nablus Jericho Tulkarm and Tubas Sera were tested by ID Screen Chlamydophila abortus Indirect Multi-species enzyme-linked immunosorbent assay ELISA kit for detection of C abortus immunoglobulin G IgG IDvet France At the individual level among 2608 samples analyzed 385 137 were seropositive for C abortus The chi-square analysis was used to evaluate the risk factors associated with regions which could provide the foundation to understand the occurrence of the diseases There was a significant difference between the regions studied P 005 Our study revealed C abortus seroprevalence rate of 177 in Nablus and Tubas cities followed by Jericho 142 Jenin 96 and Tubas 38 At the herd level among the 353 farms studied 188 533 had at least one seropositive animal The highest rate was found in the herds of Tubas city 909 followed by Nablus 783 Jenin 569 Jericho 449 and Tulkarm 364 Most of the seropositive cases were observed in cities with large herd size In conclusion detection of C abortus antibody in rams serum suggests a potential for venereal transmission that further attention should be paid to this disease in this region of the countryFirst prevalence report of contagious agalactia by Mycoplasma agalactiae in sheep in Jericho-Palestine ArticlesContagious agalactiae caused by Mycoplasma agalctiae M agalctiae implicated for important losses in small ruminant due to mortality decreased milk production and cost of treatment and prevention There is a lack of information about the disease status in Palestine In this study a survey of M agalctiae antibodies in sheep blood from Jericho city near the Jordan River in the eastern Palestine was conducted A total of 611 randomly selected sheep of different ages were investigated from different farms in two regions in Jericho city during the period from December 2016 to March 2017 to Blood samples were collected and serum was subjected to serologic examination for detection of antibodies against M agalctiae major membrane lipoprotein of p48 using enzyme-linked immunosorbent assay ELISA CHECKIT IDEXX The overall M agalctiae seroprevalence rate was 115 Regarding the distribution of the disease in the investigated regions in Jericho city; the seroprevalence rate of M agalctiae in sheep was 135 in Al-Jiftlik and 44 in Fasa\il This is the first report of M agalactiae infection in Palestine Further studies are required to determine other causeThe effect of intramuscular injection of spiramycin at therapeutic dose on some blood biochemical and hematological parameters in Assaf sheep ArticlesSpiramycin is used to treatment of different bacterial and protozoal infection in different animal species including sheep to the authors knowledge there are no studies about effects of spiramycin in sheep blood biochemical and hematological parameters This study was designated to determine the effects of spiramycin intramuscular treatment at therapeutic dose 64000 IUkg for five days in some blood biochemical and hematological parameters in healthy Assaf sheep n=8 The results showed that spiramycin treatment caused decrease in calcium and creatinine level P005 without significant change in albumin total protein magnesium glucose and the adjusted calcium level P005 After treatment hematological parameters tend to decrease toward normal references range of red blood cell count haemoglobin concentration mean corpuscular hemoglobin mean corpuscular hemoglobin concentration and red blood cell distribution width P005 No changes were observed in hematocrit mean corpuscular volume platelet count and mean platelet volume P005 All the measured biochemical and hematological parameters were in the normal references range after the treatment These results suggested that spiramycin given in therapeutic regimen to healthy Assaf sheep caused only minor inconclusive changes in the measured hematological and biochemical profiles; and thus can be used safely in treating susceptible infections in sheep These results might be accepted as a starting point for future experiments to evaluate the effects spiramycin on the different systems andparametersPresence of Aflatoxin M1 in Raw Milk for Human Consumption in Palestine Articles The absences or insufficient food control program result in the occurrence of mycotoxin in milk andmilk products which poses a serious risk for humans and can be a public health concern This study wasconducted to highlight the occurrence of aflatoxin M1 in Palestine raw milk collected at farms fromTulkarm Nablus and Jenin Aflatoxin M1 was determined by direct competitive ELISA technique 85 34 of 40 of the total examined raw milk samples tested were positive The aflatoxin M1 contaminationlevels were between 3 - 80 ppt with a mean of 2957 ppt There was a high incidence rate with 92 11of 12 and the highest means of contaminated with aflatoxin M1 in the samples tested in Tulkarm city P 005 20 of the analyzed samples 8 of 40 exceeded the maximum permissible limit 50 ppt inEuropean Codex with a range of 2 - 80 ppt Detection of β-Lactams and Tetracyclines Antimicrobial Residues in Raw Dairy Milk for Human Consumption in Palestine Articles Milk and milk products are among the most important daily consumed foodstuffs The quality andsafety of raw milk for human consumption in developed countries is not adequately monitored The lackof studies about the antimicrobial residues in milk keeps this vital topic under observation In this briefstudy 34 raw dairy milk samples were tested for detection of antimicrobial residues of -lactams andtetracyclines above Maximum Residual Limits MRLs Rapid screening test IDEXX Snap test kit wasused in this study Eighteen samples were tested for -lactams residues of which 222 4 of 18appeared to be above MRLs Sixteen samples tested for tetracycline of which 187 3 of 16 wereabove MRLs This is the first report that highlights the occurrence of antimicrobial residues marketed rawmilk for human consumption in Palestine Interaction of testosterone with inhibin a and bA subunits to regulate prostate gland growth Articles Testosterone regulation of prostate glandgrowth has been shown to involve reciprocal interactionwith inhibin and activin This study was therefore conductedto correlate the effect of testosterone on prostategland proliferation and differentiation with the level ofexpression of inhibin a and bA subunits Immature dogswere treated with testosterone for 0 3 7 and 14 days andprostate gland growth was assessed by morphological andimmunohistological localization of differentiation andproliferation markers The results showed that testosteronetreatment resulted in an initial significant increase in PCNAproliferation index by days 3 and 7 followed by a significantdecrease by day 14 post-treatment Interestingly thedecrease of cell proliferation was associated with structuraland biochemical changes characteristic of glandular andstromal differentiation of the prostate gland These changesinclude progressive glandular ductal canalization and interductalstroma differentiation which were apparent from agradual shift from vimentin expression to vimentin anda-actin expression Testosterone also had a differentialeffect on inhibin a and b subunits Although testosteronetreatment resulted in significant and constant inhibition ofa subunit mRNA expression it resulted in a significantincrease of bA mRNA expression by day 3 followed by adecrease by days 7 and 14 These results indicated thattestosterone acts first to drive proliferation of undifferentiatedprostatic cells and then to maintain a low proliferationturnover of differentiated cells Because it has beenshown that activin is an antagonistic regulator of androgensthe attenuated stimulatory effect of testosterone oncell proliferation by day 14 might be mediated at least inpart by interplay between testosterone and activin