An-Najah Blogs :: Head of Department An-Najah Blogs :: Head of Department en-us Sat, 24 Oct 2020 05:03:02 IST Sat, 24 Oct 2020 05:03:02 IST Biological effects of 6 mT static magnetic fields: a comparative study in different cell types. ArticlesTenuzzo B Chionna A Panzarini E Lanubile R Tarantino P Di Jeso B Dwikat M Dini L Department of Biological and Environmental Science and Technology University of Lecce Lecce Italy Abstract The present work was a comparative study of the bio-effects induced by exposure to 6 mT static magnetic field MF on several primary cultures and cell lines Particular attention was dedicated to apoptosis Cell viability proliferation intracellular Ca2 concentration and morphology were also examined Primary cultures of human lymphocytes mice thymocytes and cultures of 3DO U937 HeLa HepG2 and FRTL-5 cells were grown in the presence of 6 mT static MF and different apoptosis-inducing agents cycloheximide H2O2 puromycin heat shock etoposide Biological effects of static MF exposure were found in all the different cells examined They were cell type-dependent but apoptotic inducer-independent A common effect of the exposure to static MF was the promotion of apoptosis and mitosis but not of necrosis or modifications of the cell shape Increase of the intracellular levels of Ca2 ions were also observed When pro-apoptotic drugs were combined with static MF the majority of cell types rescued from apoptosis To the contrary apoptosis of 3DO cells was significantly increased under simultaneous exposure to static MF and incubation with pro-apoptotic drugs From these data we conclude that 6 mT static MF exposure interfered with apoptosis in a cell type- and exposure time-dependent manner while the effects of static MF exposure on the apoptotic program were independent of the drugs usedStatic magnetic field selects undifferentiated myelomonocytes from low-glutamine concentration stimulated U937 cells. ArticlesTenuzzo B Dwikat M Dini L Department of Biological and Environmental Science and Technology University of the Salento Via per Monteroni 73100 Lecce Italy Abstract Reduced glutamine GLN concentration in the culture medium of a U937 cell line caused them to be differentiated along the monocytic pathway; cells attached to the matrix and to each other by extending pseudopodia and acquired specific functional characteristics such as the expression of alpha-naphthyl-acetate esterase and the capacity to reduce nitroblue tetrazolium as well as becoming active phagocytes When U937 cells were differentiated under continuous exposure to a 6mT static magnetic field MF the overall differentiation process was perturbed Surprisingly after 5 days exposure to the static MF higher cell viability and differentiation were observed in cells cultured in a GLN-deprived medium than in cells grown in the same medium but in the absence of a static MF The latter cells particularly those that were still floating in the medium were stimulated with TPA for a further 3 days These cells differentiated and attached to the substrate Conversely the same treatment applied to cells cultured in GLN-deprived medium in the presence of the static MF resulted in resistance to TPA-induced differentiation Indeed these cells exhibited a round shape and in-suspension growth