An-Najah Blogs :: Plant production An-Najah Blogs :: Plant production en-us Tue, 28 Jun 2022 21:44:08 IDT Tue, 28 Jun 2022 21:44:08 IDT In vitro REGENERATION AND SOMACLONAL VARIATION OF Petunia hybrida ArticlesThe effect of different levels of naphthaleneacetic acid NAA and benzyladenine BA on shoot multiplication and regeneration of Petunia hybrida was studied Regenerated shoots from leaf explants were grown ex vitro for analysis of somaclonal variation Seeds of Petunia hybrida were germinated in vitro on MS basal medium The seedlings were used as a source of explants for the multiplication and regeneration experiments For the shoot multiplication experiment shoot explants taken from germinated seedlings were cultured on MS basal media supplemented with different concentrations of BA 01 04 08 mgl1 and NAA at concentration of 01mgl1 Highest number of axillary shoot was obtained on medium supplemented with 08 mg l-1 BA and 01 mgl1NAA For regeneration experiments leaf sections taken from germinated seedlings were cultured onto MS media supplemented with three levels of BA 05 10 and 20 mgl1 and 05 mgl1NAA The highest shoot regeneration rate 45 was observed in MS medium supplemented with 2 mgl1 BA For the evaluation of somaclonal variation lateral bud explants were taken from pink colored petunia plants that were grown in the greenhouse The buds were disinfected and cultured on MS basal media supplied with 30 mgl1 gentamycin sulfate and 30 mgl1 Benlate After shoots grew leaf sections were then taken from them shoots and cultured onto shoot regeneration medium MS medium supplemented with 2 mgl1 BA The regenerated adventitious shoots were cultured on MS medium without growth regulator These shoots were then rooted acclimatized and transferred to the greenhouse for evaluation Two forms of leaf shape orbicular and elliptic and three flower colors violet purple and light pink appeared on the plantletsEffect of Scarification, Gibberellic acid and Stratification on Seed Germination of Three Pistacia Species ArticlesGerminability of P atlantica Desf P palaestina Bioss Post and P lentiscus L seeds as influenced by various treatments was investigated This included four treatments: Acid scarification for 15 minutes soaking of acid scarified seeds in 1000 ppm GA3 for 24 hours stratification of seeds at 5Co for 30 days and control untreated seeds Significantly the highest germination 60 was obtained for P palaestina acid scarified plus cold stratified seeds over the control of the three Pistacia species 15 10 0 this percentage 60 was on the same level of significance with the other Pistacia species Scarifies seeds of P lentiscus resulted in 133 germination scarified plus GA3 soak of P lentiscus and P atlantica Desf 34 399 and scarified plus cold stratified P lentiscus 32 seeds Early seed germination was obtained with seeds of P lentiscus after one week of incubation with scarified plus GA3 6 and scarified seeds of P palaestina 5 other treated seeds of the three species started germination after two weeks P palaestina seeds continued with the highest germination percentage thereafter Significantly the longest mean time to complete germination MTG was obtained from the control of the three Pistacia species 2794 2450 30 04 Scarified P atlantica 2584 and scarified plus GA3 soak of P atlantica 2625 as compared with the other treatments for the three Pistacia species which were all in the same level of significance An-Najah University Journal for Research - Natural Sciences A ISSN: 1727-2114 Volume 21 2007 Pages: 001-011Germination of Arabequina Olive Seeds As Affected By Chemical Scarification, Hot Water Treatment And Endosperm Tissue Articles Jordan Journal of Agricultural Sciences Volume 1 No 1 2005 Direct Regeneration in Cyclamen Persicum Mill. Using ‎Seedling Tissues ArticlesIn Vitro shoot regeneration and microtuberization of Cyclamen persicum Mill were studied using seedling tissues Tuber leaf and petiole sections of aseptic seedlings of cultivar Concerto were established on Murashige and Skoog MS basal medium Three levels of benzyladenine BA 44 88 133 M and four levels of thidiazuron TDZ 05 1 2 4 M were used with the three different explants All regeneration media were supplied with 54 M naphthalene acetic acid NAA No shoot regeneration was observed in media without cytokinins The greatest percent shoot regeneration 100 was obtained from tuber sections cultured on media supplemented with 44 M BA and 4 M TDZ No regeneration was obtained with petiole sections Microtubers were formed with leaf explant The higher microtuberization response was obtained with leaf explants cultured on media supplemented with 2 and 4 M TDZ 416 and 583 respectively Tuberous structures microtubers were able to sprout and leaves continued to grow on these structures After an acclimatization period the plantlets were transferred to the greenhouse and continued their growth normally An-Najah University Journal for Research - Natural Sciences A ISSN: 1727-2114 Volume 18 Issue 2 2004 Pages: 147-156Optimal Nitrogen Fertilization for Potatoes in the West Bank-Palestine ArticlesA study was conducted in Tubas district in a field located in Wadi Al-Fara The area is under irrigation and rich with ground water Fara basin Potatoe crop was selected as it is one of the most important irrigated crops in the area Spunta cultivar was used Two experiments were conducted The first was conducted to determine the most suitable type of nitrogen source and the second experiment was conducted to determine the optimal nitrogen fertilization for potatoes It was found that the type and the amount of nitrogen fertilizer added to potatoes plants affected both crop quantity and quality The vegetative dry weight was increased when nitrogen fertilizers were used In addition the use of both urea and ammonium sulfate increases potato yield Mixing the organic acid fertilizer with ammonium sulfate or urea also increased the production insignificantly It was found that increasing the amount of nitrogen increases the number of tubers size hardness and the density of the tuber A quadratic significant relation was found between the yield and the amount of nitrogen fertilizer A maximum yield of potato under the experimental conditions was obtained with 35kgdunum nitrogen rate as ammonium sulfate Yield at this rate was 4016kgdunum Nitrate residue in the root zone at the end of the season has increased significantly when more than 20kg nitrogendunum was added When organic acid fertilizer was mixed with ammonium sulfate the nitrate was increased in the soil profile and the salt leaching was also minimized An-Najah University Journal for Research - Natural Sciences A ISSN: 1727-2114 Volume 16 Issue 2 2002 Pages: 141-154Performance of Different Grape Cultivars for Rooting and Grafting ArticlesFour local grape cultivars Halawani Beiruiti Zaini and Beituni were bench grafted on four different grape rootstocks B41 Paulsen Rugerri and Richter Two grafting methods were used: whip tongue and wedge grafting The experiment was conducted for two successive seasons 1997 and 1998 All rootstock basis were treated with Indolebutyric- acid IBA at 8000 ppm in the form of powder The results showed a great variability in the rooting ability of the different rootstocks with Paulsen rootstock which resulted in the higher rooting ability while Rugerri and B41 resulted significantly in the lower rooting percentage in both seasons The scion as well as the grafting method did not affect the rooting ability of the rootstocks The rootstock scion combination showed high healing ability with a significant interaction between both graft partners Rugerri and Richter resulted in the highest healing of grafts The vegetative bud growth of the graft varied among the different scion cultivars but without any significant effect of grafting method or rootstocks in both seasons Both Halawani and Beiruiti resulted in the higher vegetative growth during propagationThe Response of Three Varieties of Wheat to Nitrogen Fertilization ArticlesThe effects of different levels of nitrogen fertilization 0 2 4 8 and 16 kg N dunum on the production of three wheat varieties 780 Lakeesh and Anbar were studied The experiment was conducted for two successive seasons 1994 and 1995 In the second season 1995 a higher nitrogen fertilization rate 16 kg N dunum and a third variety Anbar were added The different levels of nitrogen fertilization were arranged in a Completely Randomized Design for each variety The results showed positive response of the three varieties to the nitrogen fertilization Nitrogen fertilization increased significantly the total yield and the straw weight in variety 780 and the grain weight for lakeesh variety in the first season In the second season there was a significant quadratic relation between the nitrogen fertilization and the total grain and straw weight in variety 780 and the total and grain weight for lackeesh however for Anbar variety a significant quadratic relation was only shown between the nitrogen application and straw weight There was no significant effect of nitrogen fertilization on grain size or the level of nitrogen and protein in the grains Using the yield-fertilizer application relations obtained in the experiment it was found that the optimal nitrogen fertilization which gave the highest optimal return were 57 126 and 95 kg dunum for the three varieties lakeesh 780 and Anbar subsequently The optimal amount of fertilization wasnt highly influenced with 20 changes in pricesIn Vitro Culture and Microtuberization of Spunta Potato (Solanum tuberosum L.) ArticlesDirasat Agricultural Sciences Volume 24 No 2 1997Status of date palm in Palestine ArticlesPalestine has a Mediterranean climate which is characterized by long hot dry summers and short cool rainy winters The total area of the West Bank is about 6 million donums 1 donum = 01 hectare of which 30 is cultivated while the total area of the Gaza Strip is 365000 donums of which 55 is cultivated Date palm is cultivated in a small area in the West Bank about 500 donums in Jericho and 2200 donums in Gaza The total annual production of date palm in those areas is 3000 tons This amount is about 10 of the dates consumed in the West Bank and Gaza Most of the date palms planted in Jericho are grown from seeds and called Baladi These trees produce different fruit sizes and colours with high genetic diversity Some of these trees are also old more than 50 years therefore their ability to produce offshoots has been reduced New cultivars which have been established in the area Medjool Barhee and Deglet Nour proved to grow well under Jericho conditions In Gaza 90 of date trees are of Hayani variety while the rest 10 are of other varieties Among the difficulties facing date palm culture in Jericho and Gaza are : i the limited amount of water available for agriculture; i fertilization and pollination problems; iii the lack of genetic diversity especially in Gaza; v the lack of good propagation systems; and v the lack of comprehensive planning for the agricultural sector in Gaza and JerichoThe Effect of Different Seed Treatments on Seed Germination of Gundelia tournefortii Seeds ArticlesThe effects of different seed treatments on the germination percentage and rate of Gundelia tournefortii seeds were studied Seeds were collected from Jamaeen area near Nablus city The normal healthy seeds were separated from small shrinked seed and used in the different trails Three experiments were conducted in which the effect of presoaking scarification and stratification on the seed germination was investigated The higher germination percentage 48 was obtained when the outer seed coat was removed after the seeds were presoaked in water This percentage was highly significant than other results obtained with the other treatments The use of concentrated sulfuric acid for 15 and 30 minutes to tender the seed coat didnt increase the germination percentage of the treated seeds over that of the control Similar effects was also exhibited when the seeds were soaked in hot water for different intervals Cold stratification at 4-5 didnt significantly increase the germination percentage of the seeds