An-Najah Blogs :: http://blogs.najah.edu/author/emp_2139 An-Najah Blogs :: en-us Fri, 24 Nov 2017 22:16:34 IST Fri, 24 Nov 2017 22:16:34 IST webmaster@najah.edu webmaster@najah.edu Inversion of DNA Sequences as a Result of Recombination between Site-Specific Recombination between Site-Specific Recombination Sequences from Colicin Familty Plasmidshttp://blogs.najah.edu/staff/emp_2139/article/Inversion-of-DNA-Sequences-as-a-Result-of-Recombination-between-Site-Specific-Recombination-between-Site-Specific-Recombination-Sequences-from-Colicin-Familty-PlasmidsPublished ArticlesSome naturally occurring plasmids like ColEl and ColK have been found to harbor site-specific recombination sequences the core regions of such sequences have shown 82 homology when so aligned The high degree of homology was the basis for testing the possibility of recombination between them The sequences were cloned in the vector pUC18 in opposite orientation and introduced into Ecoli cells and the analysis of the DNA harvested from the transformed cells showed that the sequences had undergone recombination such recombination such recombination was of the site-specific mode No sequence deletion was detected as was the case with such sequences when they were in the same orientation Interestingly the event of recombination in this case resulted in the inversion of the region located between the recombination sites of the two cloned fragments An-Najah University Journal for Research - Natural Sciences A ISSN: 1727-2114 Volume 14 2000 Pages: 105-116Adsorptive Stripping Voltammetric Determination of Albendazole at a Hanging Mercury Drop Electrodehttp://blogs.najah.edu/staff/emp_2139/article/Adsorptive-Stripping-Voltammetric-Determination-of-Albendazole-at-a-Hanging-Mercury-Drop-ElectrodePublished ArticlesAlbendazole is determined by differential-pulse adsorptive cathodic stripping voltammetry at a hanging mercury drop electrode using the reduction peak of its copperII complex at -028V at an accumulation potential 00V vs AgAgCl electrode The optimum conditions of pH accumulation potential and accumulation time were studied The calibration graph for the determination of albendazole was linear in the range 30X10-8 - 9X10-7M with a relative standard deviation of 28 The detection limit was 10X10-8M after 180s accumulation at 00V The effect of common excipients and metal ions on the peak height of albendazole was studied The presence of Cu2 ions forms a stable complex with albendazole which is strongly adsorbed at the mercury electrode surface The method was applied to the determination of the drug in commercially available dosage forms Analytical Letters Volume 32 Issue 15 1999 pages 2965 - 2975Multimer resolution systems of ColE1 and ColK: localisation of the crossover sitehttp://blogs.najah.edu/staff/emp_2139/article/Multimer-resolution-systems-of-ColE1-and-ColK-localisation-of-the-crossover-sitePublished ArticlesWe have identified and characterised a stability function encoded by the high copy plasmid ColK The function is analogous to ColE1 cer and maximises stability by maintaining plasmids in the monomeric state In vivo recombination between cer and ckr which share more than 90 homology at the DNA sequence level produced a functional hybrid Sequence analysis of hybrids indicates that recombination involving cer and ckr is site-specific and occurs within a 35 bp region of DNA which contains palindromic symmetry Molecular and General Genetics MGG Volume 201 Number 2 November 1985