http://blogs.najah.edu/author/bassam-abu-shanab An-Najah Blogs :: Bassam Ali Abu-Shanab en-us Sat, 20 Apr 2024 00:58:11 IDT Sat, 20 Apr 2024 00:58:11 IDT [email protected] [email protected] http://blogs.najah.edu/staff/bassam-abu-shanab/article/Enterotoxigenecity-of-s-aureus-isolates-recovered-from-chronic-urogenital-tract-infection-in-North-PalestinePublished ArticlesObjectives: Staphylococcus aureus is an important pathogen associated with diseases in a variety of hosts including humans It produces several toxins and virulence factors that contribute to its pathogenic potential such as staphylococcal enterotoxins SEs This study was conducted to determine enterotoxigenicity of S aureus associated with chronic urogenital tract infection by detecting enterotoxin genes Setting: This study was done in The Microbiology laboratory An-Najah N University Palestine Methodology: A total of 90 S aureus isolates recovered from clinical samples from patients suffering from chronic urogenital tract infection in the North of Palestine were used to detect the presence of staphylococcal enterotoxin genes sea seb sec sed and see by polymerase chain reaction PCR assay Results: Out of 90 S aureus isolates tested it was found that 57 633 of these isolates harboured one or more enterotoxin genes Up to 789 of the enterotoxigenic isolates possessed one SE gene The majority of these enterotoxigenic strains 614 isolated from both semen and urine samples harbored sec gene either alone or in combination with other genes Also the prevalence of genes in combination was significantly more common in S aureus isolates derived from urine 933 273 as compared to those derived from semen 324 125 Conclusions: The role of enterotoxin genes in the pathogenesis of urogenital tract infection is still unknown However it is evident that urogenital infection can be caused by S aureus strains which lack these genes Other newly detected genes may play a role in pathogenesishttp://blogs.najah.edu/staff/bassam-abu-shanab/article/In-Vitro-Activity-of-Certain-Drugs-in-Combination-with-Plant-Extracts-against-Staphylococcus-Aureus-InfectionsPublished ArticlesObjectives: This study has been done to evaluate the interaction between ethanolic extracts of Rhus coriaria seed Psidium guajava Leaf Lawsonia inermis Leaf and Sacropoterium spinosum seed and antimicrobial drugs including oxytetracycline HCl enrofloxacin gentamicin sulphate and sulfadimethoxine against four clinical isolates of methicillin-resistant Staphylococcus aureus MRSA Methodology: Evaluation of the interaction between ethanolic extracts and different antimicrobial agents has been done using well-diffusion method Results: It showed that ethanolic extracts increase the inhibition zones of oxytetracycline HCl gentamicin sulphate and sulfadimethoxine while combinations between these plant extracts and enrofloxacin decrease inhibition zone Conclusion: This study probably suggests the possibility of concurrent use of these antimicrobial drugs and plant extracts in combination in treating infections caused by S aureus strains or at least the concomitant administration may not impair the antimicrobial activity of these antibioticshttp://blogs.najah.edu/staff/bassam-abu-shanab/article/Efficacy-of-Aqueous-and-Ethanol-Extracts-of-Some-Palestinian-Medicinal-Plants-for-Potential-Antibacterial-ActivityPublished ArticlesNine medicinal plants growing in Palestine were screened in vitro for potential antibacterial activity against 6 bacterial strains by well diffusion and micro-dilution techniques Both aqueous and organic solvents were used The dried extracts of Sacropoterium spinosum Rosaceae seed Ruta chalepensis L Rutaceae leaf Cassia senna Ligumenosa leaf Lawsonia inermis Lythraceae leaf Psidium guajava Myrtaceae Leaf Carataegus azerullus Rosaceae Leaf Ranunclus asiaticusRanunculaceae Flowers Calendula officinalis Composita Flowersand Salvia syriaca Labiatae leaf were screened The bacterial strains tested were; Methicillin-resistant Staphylococcus aureus MRSA; three strains 1 2 3 multidrug resistant Pseudomonas aeruginosa Proteus vulgaris and Klebsiella pneumonia The average diameter of inhibition zones ranged from 9 to 30 mm and 11 to 28 mm for aqueous and ethanol extract respectively Methicillin-resistant Staphylococcus aureus MRSA was the most inhibited microorganism Sacropoterium spinosum extract was the most active against Methicillin-resistant Staphylococcus aureus MRSA and multidrug resistant Pseudomonas aeruginosa The MIC value of ethanol extract was 0781 mgml against MRSA while 0390 mgml against Pseudomonas aeruginosa The combination effect of ethanol extracts of Sacropoterium spinosum with Lawsonia Inermis on bacterial species tested exhibited a higher effect than that of any individual extract Such results lead to an interesting promise for further investigation to design potentially active antibacterial augmentative agents of natural sources Key words: Antibacterial activities plant extracts methicillin resistant Staphylococcus aureus multi-drug resistant Pseudomonas aeruginosa Palestinehttp://blogs.najah.edu/staff/bassam-abu-shanab/article/Antibacterial-Effects-of-Nutraceutical-Plants-Growing-in-Palestine-on-Pseudomonas-aeruginosaPublished ArticlesThe antibacterial effects of single and combined plant extracts of water ethanol and methanol for two nutraceuticals utilized in Palestine were studied against multiple drug resistances Pseudomonas aeruginosa using well diffusion method These plants are Rhus coriaria and Thymus vulgaris Combinations of these extracts showed an additive action against this pathogenhttp://blogs.najah.edu/staff/bassam-abu-shanab/article/Antibacterial-Activity-of-Four-Plant-Extracts-Used-in-Palestine-in-Folkloric-Medicine-against-Methicillin-Resistant-Staphylococcus-aureusPublished ArticlesEthanolic and hot water extracts from 4 different plant species used in Palestine in popular medicine for the treatment of several ailments of microbial and non-microbial origin were evaluated for potential antimicrobial activity against methicillinresistant Staphylococcus aureus MRSA Both water and ethanol extracts of Mentha longifolia Melissa officinalis and Rosa damascena were effective on MRSA The minimum inhibitory concentration MIC and minimum bactericidal concentration MBC values of the ethanolic extract of M longifolia and M officinalis were in the range of 3125 to 1250 mgml and 1250 to 2500 mgml respectively The ethanolic extract with the greatest antimicrobial activity was that of R damascena MIC 0395 to 0780 mgml and MBC 1563 to 3125 mgml The combination of ethanolic extracts of the plants studied showed synergistic antibacterial activity against MRSA strainshttp://blogs.najah.edu/staff/bassam-abu-shanab/article/Toxigenicity-of-Staphylococcus-aureus-isolates-from-Northern-PalestinePublished ArticlesA total of 68 Staphylococcus aureus strains isolated from different human clinical samples in the North of Palestine were examined to detect staphylococcal enterotoxin SE genes A sea B seb C sec D sed and see Of the total isolates examined 412 2868 were enterotoxigenic S aureus Twelve strains 429 of enterotoxigenic S aureus harbored seagene ten strains 357 were carried see- gene six strains 214 were positive for sec-gene None of these enterotoxigenic S aureus isolates harbored more than one of toxin genes The presence of these toxin genes and other genes not be detected here might play a role in process of pathogenesis of S aureus disease other than food poisoning but this cannot be substantiated by the results of the present study Emirates Medical Journal 2006; 242http://blogs.najah.edu/staff/bassam-abu-shanab/article/Antibacterial-Activity-of-Rhus-Coriaria-L-Extracts-Growing-in-PalestinePublished ArticlesDisk diffusion and micro-dilution techniques were used to determine the antibacterial activity of water ethanolic and methanolic extracts of Rhus coriaria ripe berries against five clinical bacterial strains Methicillin-resistant Staphylococcus aureus MRSA multi-drug resistant Pseudomonas aeruginosa enterohemorrhagic Escherichia coli O157 EHEC Proteus vulgaris and Klebsiella pneumoniae Bacillus subtilis ATCC6633 was used as a reference strain The zone of inhibition varies depending on bacterial species and type of extract The average diameter of inhibition zones ranges from 0 to 19 mm 15 to 25 mm and 15 to 22 mm for water ethanolic and methanolic extract respectively The MIC value of ethanolic extract was 125 mgml against MRSA EHEC P aeruginosa and Proteus vulgaris while 0156 mgml against B subtilis In general these results showed that the antibacterial activity of R coriaria ripe berries extracts was more effective against Gram-positive bacteria than Gram-negativehttp://blogs.najah.edu/staff/bassam-abu-shanab/article/Nosocomial-Infection-Caused-by-Methicillin-Resistant-Staphylococcus-aureus-in-PalestinePublished ArticlesThis report presents the prevalence of Palestinian isolates of methicillin-resistant Staphylococcus aureus MRSA in nosocomial infections and their antibiotic resistant pattern A total of 321 clinical isolates of S aureus were identified from different patients The prevalence of methicillin resistance among S aureus isolates was 87 28 isolates Resistance rates of MRSA to other antibiotics were as follows: 821 resistant to erythromycin 679 to clindamycin 643 to gentamicin and 321 to ciprofloxacin No co-trimoxazole- and vancomycin-resistant isolates were identified in this study The proportion of methicillin resistance was highest among S aureus isolates associated with upper respiratory specimens 428; the proportion of methicillin resistance was 393 among skin ulcer isolates 107 among urinary tract infection isolates and lowest among isolates associated with blood and prostate discharge 36 each http://blogs.najah.edu/staff/bassam-abu-shanab/article/Enterotoxigenic-Staphylococcus-aureusin-Raw-Milk-in-the-North-of-PalestinePublished ArticlesEnterotoxin genes sea-see in Staphylococcus aureus isolates recovered from milk of clinically healthy sheep and cows in the north of Palestine were determined using a polymerase chain reaction PCR Thirty-seven 37 out of 100 S aureusisolates were toxin gene positive Four strains 108 were sea-positive 20 541 were seb-positive 4 108 were sec-positive 6 162 were sed-positive and 3 81 were see-positive None of these enterotoxigenic isolates carried more than one toxin gene This study indicates that the presence of enterotoxigenic S aureusin raw milk can contribute to the sources of staphylococcal food poisoning in Palestinehttp://blogs.najah.edu/staff/bassam-abu-shanab/article/Molecular-epidemiology-of-antibiotic-resistant-Escherichia-coli-isolated-from-hospitalized-patients-with-urinary-tract-infections-in-Northern-PalestinePublished ArticlesEighty isolates of Escherichia coli were collected in Northern Palestine throughout the 1996 to 2000 period from hospitalized patients with urinary tract infections UTIs Resistance rates were ampicillin 65; co-trimoxazole 55; cefuroxime 10; cefotaxime 75; ceftazidime 25; ciprofloxacin 125; gentamicin 625 and amikacin 125 No imipenem-resistant isolates were identified To determine whether this was due to intra-hospital transmission of resistant strains clonal structure of 10 multiple-resistant isolates was examined by genomic DNA fingerprinting by enterobacterial repetitive intergenic concensus-polymerase chain reaction ERIC-PCR and all were clonally distinct Thus these strains are likely resistant due to convergent acquisition of resistance determinants by genetically unrelated uropathogenic strains rather than epidemic spread of resistant isolateshttp://blogs.najah.edu/staff/bassam-abu-shanab/article/Antibacterial-Activities-of-Some-Plant-Extracts-Utilized-in-Popular-Medicine-in-PalestinePublished ArticlesThe antibacterial activities of hot water methanol and ethanol extracts of 5 plant extracts utilized in Palestine in popular medicine were studied The dried extracts of Syzyium aromaticum Myrtaceae seed Cinnamomum cassiaLauraceae cassia bark Chinese cinnamon bark Salvia officinalis Lamiaceaea leaf Thymus vulgaris Lamiaceaea leaf and Rosmarinus officinalisLabiatae leaf were tested in vitro against 4 bacterial species by disk diffusion and micro-dilution The patterns of inhibition varied with the plant extract the solvent used for extraction and the organism tested Methicillin-resistant Staphylococcus aureusMRSA and Bacillus subtilis ATCC 6633 were the most inhibited microorganisms S aromaticumextract was the most active against multidrug resistant Pseudomonas aeruginosaand enterohemorrhagic Escherichia coli O157 EHEC The combinations of ethanolic extracts of S officinalis with R officinalisand of R officinalis with T vulgaris on bacterial species tested exhibited a higher effect than that of any individual extract Results of this kind herald the interesting promise of designing a potentially active antibacterial synergized agent of plant origin