An-Najah Blogs :: Amjad I. A. Hussein An-Najah Blogs :: Amjad I. A. Hussein en-us Fri, 01 Jul 2022 01:25:28 IDT Fri, 01 Jul 2022 01:25:28 IDT Multiple-subgenotype infections of Giardia intestinalis detected in Palestinian clinical cases using a subcloning approach Articles To evaluate the geographic distribution of G intestinalis genotypes in Nablus West Bank Palestine a genotyping study was performed using clinical fecal samples Microscopic examination confirmed that 8 of 69 116 samples were G intestinalis positive and subsequent genotyping analyses targeting the small-subunit ribosomal RNA 18S rRNA and glutamate dehydrogenase GDH genes revealed the G intestinalis genotypes within the 8 samples Of these 8 samples 6 were clustered with assemblage A-II and the remaining 2 samples were clustered with assemblage B by 18S rRNA gene analysis; however direct sequencing of the GDH gene segments from the latter 2 samples showed a mixed infection profile To assess those samples we employed a subcloning approach and successfully isolated 6 independent assemblage B subgenotypes These partial GDH gene sequences 393 bp had 15 single-nucleotide polymorphisms all of which were synonymous transition substitutions at the third nucleotide position of codons From the results we concluded that the highly polymorphic gene loci such as GDH gene locus might provide us an opportunity to obtain a detailed molecular data even from the samples with multiple-subgenotype mixed infections Therefore subcloning approach is recommended in genotyping studies especially in those conducted in giardiasis-endemic areas where the repeated and cumulative infections could be commonly expected